Colistin Susceptibility Testing Using the MicroScan® Colistin Well

Colistin Susceptibility Testing Using the MicroScan® Colistin Well

Colistin susceptibility testing: time for a review.

respectively, relative to the S. maltophilia strain IAM 1566 protein) (Figure 1). S. maltophilia is a Gram-negative bacterium found in a variety of environments, including soil, water and plants, and is therefore a potential reservoir of the MBL gene. Similar to POM-1 and L1 MBLs, the ability of the PAM-1 enzyme to hydrolyse carbapenems might be relatively low; consequently, the PAM-1-positive ...

Pharmacokinetics of colistin methanesulphonate and colistin in rats following an intravenous dose of colistin methanesulphonate.

OBJECTIVES To determine the disposition of colistin methanesulphonate (CMS) and colistin following intravenous (iv) administration of CMS in rats. METHODS Five rats received a single iv bolus of 15 mg/kg CMS. Plasma concentrations of CMS and of colistin formed by the hydrolysis of CMS were determined by HPLC. The pharmacokinetic parameters of CMS and colistin were calculated using non-compart...

Studies on the selectivity of action of colistin, colistin nonapeptide and colistin heptapeptide on the cell envelope of Escherichia coli.

KOIKE e t al. reported that a cationic acylpeptide antibiotic, colistin (polymyxin E) caused the formation of blebs on the outer layer of the Gram-negative bacterial cell envelope". They have also reported that colistin affects the cytoplasmic membrane, leads to leakage of cytoplasmic 260 nm-absorbing materials and has specific bursting-activity on spheroplasts of Gram-negative bacteria'). On t...

Colistin and Polymyxin B Susceptibility Testing for Carbapenem-Resistant and mcr-Positive Enterobacteriaceae: Comparison of Sensititre, MicroScan, Vitek 2, and Etest with Broth Microdilution.

Colistin and polymyxin B remain part of the last line of antibiotics for multidrug-resistant Gram-negative bacteria, such as carbapenem-resistant Enterobacteriaceae Current joint EUCAST-CLSI recommendations are for broth microdilution (BMD) to be performed for MIC testing of colistin. Commercial susceptibility testing methods were evaluated and compared against the reference BMD, using a suscep...